Abstract
A reliable and highly sensitive hydrogen peroxide (H2O2) field effect transistor (FET) sensor is reported, which was constructed by using molybdenum disulfide (MoS2)/reduced graphene oxide (RGO). In this work, we prepared MoS2 nanosheets by a simple liquid ultrasonication exfoliation method. After the RGO-based FET device was fabricated, MoS2 was assembled onto the RGO surface for constructing MoS2/RGO FET sensor. The as-prepared FET sensor showed an ultrahigh sensitivity and fast response toward H2O2 in a real-time monitoring manner with a limit of detection down to 1 pM. In addition, the constructed sensor also exhibited a high specificity toward H2O2 in complex biological matrix. More importantly, this novel biosensor was capable of monitoring of H2O2 released from HeLa cells in real-time. So far, this is the first report of MoS2/RGO based FET sensor for electrical detection of signal molecules directly from cancer cells. Hence it is promising as a new platform for the clinical diagnosis of H2O2-related diseases.
Highlights
H2O2 released from living cells is important for biological and clinical diagnostics application
We have prepared a high performance field effect transistor (FET) sensor with the introduction of MoS2 and reduced graphene oxide (RGO) for highly sensitive and specific detection of H2O2 from cancer cell, in which the MoS2 nanosheets is employed as the catalytic layer and RGO is used as the conductive layer
The above-mentioned experiment results prove that the observed current response came from H2O2 released from the HeLa cells. These results firmly demonstrate that the constructed MoS2/RGO FET sensor was capable of real-time monitoring H2O2 released from living cells
Summary
H2O2 released from living cells is important for biological and clinical diagnostics application. 2D sheet-like structure of transition metal dichalcogenides (TMDCs) has attained significant amount of interest due to their potential applications in nanoelectronics, sensing, and energy harvesting Among these 2D TMDCs nanosheets, molybdenum disulfide (MoS2) is a naturally lamellar material with three atom layers (S-Mo-S) stacked together to form a sandwich structure. Lei et al.[22] utilized excellent peroxidase-like activity of few layer MoS2 for the colorimetric detection of H2O2 with high sensitivity. We have prepared a high performance FET sensor with the introduction of MoS2 and reduced graphene oxide (RGO) for highly sensitive and specific detection of H2O2 from cancer cell, in which the MoS2 nanosheets is employed as the catalytic layer and RGO is used as the conductive layer. The MoS2/RGO FET biosensor is applied to monitor trace amount of H2O2 released from cancer cells
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