Abstract
This paper describes a simple, rapid and sensitive chemiluminescence (CL) method for the determination of perphenazine by flow injection (FI) system. All variables that can affect the CL response were optimized by employing central composite design (CCD) for the experimental design and response surface methodology (RSM) for the modeling. Optimization by means of CCD method with respect to conventional single factor at a time method showed a significant improvement in the sensitivity. Under the optimum experimental conditions, a wide linear relationship between analyte concentration and peak height was obtained within the range 1.2-1,300 ng mL-1 with correlation coefficient of 0.9978. The limit of detection was 0.4 ng mL-1 (S/N = 3) and the relative standard deviation for 6 repeated measurements of a solution containing 70.5 ng mL-1 was lower than 4%. This method was successfully applied for the quantification of perphenazine in pharmaceutical formulations and human serum with good recoveries (95.3-104.0%). Sample throughput was 100 ± 5 samples per hour.
Highlights
Perphenazine belongs to the phenothiazine family of drugs
Analysis of variance (ANOVA) of the results showed that Ce(IV) and H2SO4 concentrations along with interaction between them were important terms and they had a statistically significant effect on the CL Intensity (p < 0.05 at a 95% confidence level)
The results show that a relatively wide linear dynamic range is obtained in the range of 1.2‐1,300 ng mL–1 which its linear equation was: ICL = 0.0011(± 0.0000) CPerphenazine – 0.0003 (± 0.0001), In order to evaluate the applicability of the proposed method, two perphenazine drugs and spiked human serum were analyzed to determine their perphenazine contents
Summary
Perphenazine belongs to the phenothiazine family of drugs. Because of its neuroleptic and antidepressive actions, it is often prescribed for the treatment of psychotic patients such as schizophrenia and schizoaffective psychoses in order to decrease restlessness, aggressiveness and impulsive behavior. It is found to be effectual in the treatment of Parkinson’s disease.[1] The monitoring of the perphenazine is important for quality assurance in pharmaceutical industry and for obtaining optimum therapeutic concentrations in body fluids to minimize the risk of toxicity. The. Chemiluminescence (CL) is becoming a powerful analytical tool due to its high sensitivity, wide linear dynamic range and simple instrumentation.[18,19] The CL involving [Ru(bpy) ]2+ is one of the most interesting series of CL reactions. Chemiluminescence (CL) is becoming a powerful analytical tool due to its high sensitivity, wide linear dynamic range and simple instrumentation.[18,19] The CL involving [Ru(bpy) ]2+ is one of the most interesting series of CL reactions
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call