Determination of melamine by flow injection analysis based on chemiluminescence system

Abstract

In this paper, based upon the phenomenon that melamine can obviously enhance the CL signal of the luminol–H2O2 system in basic medium, a simple, rapid and sensitive flow injection chemiluminescence (FI-CL) method for the determination of melamine has been developed. Under the optimum conditions, the linear range for the determination of melamine was 0.2–80μgmL−1 with a detection limit of 0.12μgmL−1 calculated as proposed by IUPAC and a relative standard deviation of 3.26% for 11 solutions of 10μgmL−1 melamine on the same day. The proposed method was satisfactorily applied to determine melamine in milk-based products and satisfactory results were obtained without interferences from the sample matrix. Moreover, one assay produce takes only 25s and the minimum sampling rate is about 120 samplesh−1, which indicated that the FI-CL method was suitable for high throughput and real-time melamine analysis.