Abstract
Despite the success achieved in the comprehensive study of probiotic cultures, today there are a number of problems associated with the low viability of lactic acid bacteria during their processing and long-term storage in probiotics. Our work aimed to select the optimal technological regime and cryoprotectant to preserve the viability of lactic acid bacteria Lactobacillus spp. during their lyophilization. According to the results of the conducted researches, it is established that for freeze-drying of probiotic cultures Lactobacillus spp. in the facility LZ-45.27 (Frigera, Czech Republic) the most optimal is the mode which provides a rise of temperature within 45 hours from minus 70.0±1.0 °C to plus 26.0±1.0 °C with a speed of 2.2±0.1 °C/hour. It is effective to use protective media for lactobacilli, which consist of: skim milk (90%) and sucrose (10%); skim milk (90%) and lactose (10%); skim milk (90%), glucose (2.5%), sucrose (2.5%), lactose (5.0%) (P≤0.05). Freeze-drying of lactic acid bacteria under optimal conditions and the addition of cryoprotectants will avoid the problems associated with a significant reduction in the number of microbial cells. The results of research can be used for long-term storage of cultures of lactobacilli by their lyophilization.
Highlights
Today in veterinary medicine in the general complex of preventive and therapeutic measures much attention is paid to the use of environmentally friendly, natural means of animal protection (Paliy et al, 2018; Nazarenko et al, 2020; Rodionova et al, 2020; Kasianenko et al, 2020), which include probiotic drugs
The viability of lactobacilli was determined by comparing the number of colony-forming units (CFU) in 1 cm3, before and after freezedrying, and the use of cryoprotectants by serial dilutions of the resulting suspension in saline followed by seeding on agar medium MPC-4
Sucrose, and lactose were used as cryoprotectants for the protective medium, separately at concentrations of 10.0%, and their composition at a concentration of 2.5%; 2.5%; 5.0% of each ingredient, respectively, which were added to skim milk
Summary
Today in veterinary medicine in the general complex of preventive and therapeutic measures much attention is paid to the use of environmentally friendly, natural means of animal protection (Paliy et al, 2018; Nazarenko et al, 2020; Rodionova et al, 2020; Kasianenko et al , 2020), which include probiotic drugs. Different species of lactobacilli are found in nutrient-rich habitats associated with food, feed, plants, animals and humans (Duar et al, 2017). The genus of lactic acid bacteria Lactobacillus spp. has more than 130 different species and is represented by non-pathogenic, gram-positive obligate or facultative anaerobes with high enzymatic activity (Talib et al, 2019). Most lactobacilli are part of the normal microflora of the gastrointestinal tract and are represented mainly by Lactobacillus acidophilus, L. casei, L. bulgaricus, L. plantarum, L. salivarius, L. reuteri and L. rhamnosus (Paliy et al, 2020b). The antimicrobial action of lactic acid bacteria against rotaviruses (Liévin-Le Moal & Servin, 2014), yeast (Coman et al, 2014), food and feed filamentous fungi (Prema et al, 2010) has been established. Most strains of L. plantarum produce β-galactosidase and bile salt hydrolase, which improve the overall condition of the macroorganism (Shekh et al, 2016)
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