Abstract
Recently, a Salmonella Typhi isolate producing CTX-M-15 extended spectrum β-lactamase (ESBL) and with decreased ciprofloxacin susceptibility was isolated in the Democratic Republic of the Congo. We have selected bacteriophages that show strong lytic activity against this isolate and have potential for phage-based treatment of S. Typhi, and Salmonella in general.
Highlights
The fourteen phage clones were isolated from the river Mtkvari in Tbilisi, from the
The host range of the phages was assessed by screening their lytic activity and using the spot test against 118 clinical and 121 veterinary Salmonella. spp. isolates from Georgia (20), Armenia (71), Germany (7), and Ireland (141)
Typhi isolate without forming phage-resistant mutants after 24 h of incubation, which is indicative of their inherent ability to limit the growth of phage-resistant mutants during phage therapy: GE_vB_N3, GE_vB_N5, GE_vB_N8, GE_vB_NS7 and GE_vB_HIL (Table 1)
Summary
The fourteen phage clones were isolated from the river Mtkvari in Tbilisi, from the The host range of the phages was assessed by screening their lytic activity and using the spot test against 118 clinical and 121 veterinary Salmonella. After the plates had cooled, 5 μL of the phage clones, with a titer of 107 plaque forming units (pfu)/mL was spotted on the lawn.
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