Abstract
The aim of this study is to find and use effective IRAP markers for DNA genotyping of representatives of sakura species (ornamental cherries). The work demonstrates the results of testing ISSR markers on the genotypes of the species P. serrulata. Also, based on the testing data, the selection of effective IRAP markers was carried out. The selected IRAP markers were used in the genetic analysis of 12 sakura genotypes.
Highlights
The practice of using multilocus markers as tools for DNA certification has been widely introduced into genetic research
The aim of this study is to find and use effective IRAP markers for DNA genotyping of representatives of sakura species
The carried out research work allowed to identify effective IRAP markers for genotyping of decorative cherries - sakura
Summary
The practice of using multilocus markers as tools for DNA certification has been widely introduced into genetic research. IRAP marker polymorphism nature consists in the variability of the genome regions localized between the insertion sites of a the same type retrotransposons [1,2]. IRAP markers are genomic regions bounded by retrotransposon insertions on both sides. In the course of IRAP labeling, one variant of the primer is used in the PCR mixture, which is complementary to the termination of the particular retrotransposons LTR region [8]. In this connection, the IRAP sequence in PCR acts as a forward and reverse primer, flanking the target DNA fragments from both sides [9]. The above-mentioned features of this method make it a convenient approach in genetic studies of cultures at the initial stage [11,12]
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