Abstract
Centrifugal ultrafiltration and LC-MS methods were combined to screen DNA-binding agents from traditional Chinese medicine red clover isoflavone extracts. The results show that 10 red clover isoflavones have different binding degrees with DNA. The sample was analyzed by LC-MSn for identifying structures. Among these flavones studied, the strongest five potential DNA-binding agents are irilone-4(i)-O-beta-D-glucoside, glycitin, pratensein, irilone and biochanin A, respectively. A rapid centrifugal ultrafiltration-LC-MS platform for screening and identification of DNA binders from the herbal extract and other complex system was established.
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