Screening and identification of mRNAs targeted by human cytomegalovirus (HCMV)-encoded miR-US33-1-5p in HCMV-infected human embryo lung fibroblast cells

Abstract

Objective To screen and identify mRNAs targeted by human cytomegalovirus-encoded miR-US33-1-5p (HCMV miR-US33-1-5p) for understanding the biological functions of HCMV miR-US33-1-5p. Methods Potential target mRNAs of HCMV miR-US33-1-5p were screened out by using hybrid-PCR, a simple and effective method. Dual-Luciferase Reporter Assay System was used to identify the binding abilities of HCMV miR-US33-1-5p to 3′-UTRs of these potential target mRNAs. Results Twelve potential mRNAs targeted by HCMV miR-US33-1-5p were screened out in human embryo lung fibroblast cells infected with HCMV. Luciferase activities of 3′-UTRs of seven target mRNAs were significantly down-regulated. Conclusion Proteins encoded by these target mRNAs are involved in virus replication, immune system regulation, protein synthesis, cell cycle regulation, energy metabolism and so on. Identification of mRNAs targeted by HCMV miR-US33-1-5p is helpful for further studies on biological functions of miR-US33-1-5p. Key words: Human cytomegalovirus; miRNA; Target mRNA