Abstract
Objective To discuss selective killing effects of recombinant adenovirus vectors of cytosine deaminase(CD) gene using a human telomerase reverse transcriptase (hTERT) promoter-driven system on cervical cancer cells. Methods Hela cells(study group) and human embryo lung fibroblast (hELF) cells(control group) were separately infected with recombined adenovirus. Then different titers of recombinant adenovirus and different concentrations of 5-flurocytosine acting on Hela cells were performed to measure cell viability and killing effects of adenovirus vector on two groups. Results After transfection by recombinant adenovirus Ad-hTERTp-CD in vitro, and combined with 5-flurocytosine, the cell growth of Hela cancer cells(study group) were suppressed compared with human embryo lung fibroblast cells(control group) (P<0.01). Conclusion Recombinant adenovirus combined with 5-flurocytosine has a significant killing effect on Hela cells in vitro, but not on human embryo lung fibroblast cells. Key words: human telomerase reverse transcriptase (hTERT); suicide gene; cytosine deaminase gene; adenovirus vector
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