Schizophrenia is not associated with changes in blood expression levels of complement factors B, H and I

Abstract

Event Abstract Back to Event Schizophrenia is not associated with changes in blood expression levels of complement factors B, H and I Hovsep Ghazaryan1, 2*, Anna Boyajyan1, Roksana Zakharyan1, Ani Melkonyan1, Zdenka Navratilova2, Petra Klevcova2 and Martin Petrek2 1 Institute of Molecular Biology, National Academy of Sciences of the Republic of Armenia, Armenia 2 Institute of Molecular and Translational Medicine, Faculty of Medicine and Dentistry, Palacky University, Czechia Our previous studies indicated systemic hyperactivation of the alternative complement cascade in schizophrenia. However, molecular pathomechanisms of detected alterations are not clear yet. In the present study we evaluated the expression levels of factor B, H and I in peripheral blood of patients with schizophrenia (n=66) compared to healthy controls (n=99). Factor B is an essential component of the alternative pathway. Factors H and I are negative regulators of this pathway. Methodological design includes quantitative real-time polymerase chain reaction (RT-PCR) of mRNA samples isolated from the peripheral blood cells of the study subjects. Our results showed no significant changes in the selected proteins mRNA expression were detected in patients compared to controls (p>0.05). In summary we concluded that hyperactivation of the complement alternative pathway is most probably not conditioned by changes in the expression levels of factor B, factor H and factor I in the peripheral blood. Acknowledgements We thank the administration and medical staff of the clinics of Psychiatric and Erebouni Medical Centers of the Ministry of Health of the Republic of Armenia for selection of patients and healthy subjects. This study was supported by the International Visegrad fund scholarship (HG51200594), the National Academy of Sciences of Armenia, IGAPULF2013_009 and CZ.1.05./2.1.00/01.0030 grant funding. Keywords: Schizophrenia, mRNA, CFB, CFH, CFI Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Ghazaryan H, Boyajyan A, Zakharyan R, Melkonyan A, Navratilova Z, Klevcova P and Petrek M (2013). Schizophrenia is not associated with changes in blood expression levels of complement factors B, H and I. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00351 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 21 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Mr. Hovsep Ghazaryan, Institute of Molecular Biology, National Academy of Sciences of the Republic of Armenia, Yerevan, Armenia, h_ghazaryan@mb.sci.am Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hovsep Ghazaryan Anna Boyajyan Roksana Zakharyan Ani Melkonyan Zdenka Navratilova Petra Klevcova Martin Petrek Google Hovsep Ghazaryan Anna Boyajyan Roksana Zakharyan Ani Melkonyan Zdenka Navratilova Petra Klevcova Martin Petrek Google Scholar Hovsep Ghazaryan Anna Boyajyan Roksana Zakharyan Ani Melkonyan Zdenka Navratilova Petra Klevcova Martin Petrek PubMed Hovsep Ghazaryan Anna Boyajyan Roksana Zakharyan Ani Melkonyan Zdenka Navratilova Petra Klevcova Martin Petrek Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.