Abstract
Abstract The isotopic composition of water extracted from various parts of alfalfa (Medicago saliva) plants was compared with that transpired by the plants to validate water extraction procedures, and to define the degree of alteration of the isotopic composition of water that occurred in different plant parts. The mean δ2H and δ18O values of water extracted from the upper tap root and lower crown regions were < 1‰ and 0.3‰ different from that of water transpired, respectively, with an analytical precision similar to that found in previous studies on soils and trees. Water from other plant parts closer to the leaves was enriched in 2H (up to 12.6 in upper stems) and 18O (4.2‰) with respect to that transpired. The enrichment was associated with a greater variability in δ2H values between replicate plants. δ2H‐δ18O data indicated that the isotopic enrichment was due to evaporation during transpiration. The study showed that azeotropic distillation is an accurate means of extracting water from alfalfa, altho...
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