Abstract
This study evaluated the combined impact of salinity (2.5, 13, and 19.3 g NaCl/L) and inoculum pretreatment (acid/alkali) on the genomic and metabolic profiles of mesophilic fermentative bacteria for hydrogen (H2) production from food waste. Experimental results revealed that acid-treated inoculum showed the highest H2 yield (201.12 ± 13.84 mL H2/g of volatile solids added) under medium salinity levels compared to other experimental conditions. A 7–56% increase in H2 yield was observed for pretreated inoculum than untreated inoculum. Genomic analysis and metabolic pattern revealed that the H2 production was mainly through Clostridial-type fermentation under medium to high salinity levels, whereas Enterococcus-type fermentation under low salinity levels. Further, the genomic analysis uncovered that phyla Firmicutes (69.71–96.81%) and genus Clostridium sensu stricto 1 (33.28–94.04%) dominated during the exponential gas production phase. Overall, this study showed the significance of inoculum pretreatment for the bioconversion of food waste at different salinity levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
