Rutin and Vitamin E alleviate oxidative stress and hepatic-renal injury induced by technical and formulated Lambda-cyhalothrin in adult rats

Abbassy Moustafa Abdellatif Abbassy Moustafa Abdellatif,Mansy Awatef S Mansy Awatef S,Nasr Hoda M Nasr Hoda M

doi:10.30574/gscbps.2020.12.1.0204

Abbassy Moustafa Abdellatif Abbassy Moustafa Abdellatif, Mansy Awatef S Mansy Awatef S + Show 1 more

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https://doi.org/10.30574/gscbps.2020.12.1.0204

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Abstract

Lambda-cyhalothrin (LCT) is a widely used pyrethroid to control insect pests in agriculture, public health and homes. The present study was designed to assess some of the toxicological effects in male rats following exposure to technical and formulated lambda-cyhalothrin (LCT T and LCT F.) and to evaluate the ameliorative effect of Vitamin E and Rutin administration. Nine groups of rats were designated for: control group; RT (50 mg kg-1 b.wt.); Vitamin E (100 mg kg-1 b.wt.); LCT T. (20 mg kg-1 b.wt); [LCT T. +Vitamin E]; [LCT T. +Rutin]; LCT F. (20 mg a.i. kg-1 b.wt); [(LCT F.) +Vitamin E] and [(LCT F.) +Rutin]. Results revealed that both of technical and formulated lambda-cyhalothrin treatments caused significant increase in LPO level and decrease in the activities of antioxidant enzymes: catalase, superoxide dismutase, glutathione-s-transferase Glutathione peroxidase, and glutathione reductase. Also, both forms of lambda-cyhalothrin caused significant increase in the activity of alanine and aspartate transaminases and alkaline phosphatase, lactate dehydrogenase and creatinine, uric acid levels, whereas albumin, total protein were decreased. Coadministration of Vit.E or Rutin achieved noticeable ameliorative effects. Thus, the overall results revealed the powerful ameliorating effect of Vit.E and Rutin in pyrethroids (e.g.LCT)-intoxicated rats.

Highlights

Pyrethroids are among the most widely used synthetic pesticides
There was no significant changes in the activity of the biomarker enzymes alanine transaminase (ALT), AST, Alkaline phosphatase (ALP) and Lactate dehydrogenase (LDH) in the serum of Rutin - or Vit E - treated rats compared to control, while λ-Cyhalothrin- (LCT T., LCT F.) treated rats showed significant increase (P≤ 0.05) in these biomarkers, indicating the occurrence of hepatic injury (Table1).co-treatment with Vit.E or RT restored the levels of these biomarkers in the combined groups [(LCT.T) + Vit.E}, [(LCT.T) + RT], [(LCT.F) + Vit.E] and [(LCT.F) + RT] back to normal control
Serum superoxide dismutase (SOD), CAT, Glutathione peroxidase (GPx), glutathione reductase (GR), GST activites were significantly decreased in LCT-treated rats, while serum lipid peroxidation (LPO) level was significantly (P ≤ 0.05) increased as compared with control group

Summary

Introduction

Pyrethroids are among the most widely used synthetic pesticides Their widespread use has stimulated research into the possible existence of effects related with their oxidative stress and hepatic-renal toxicity in mammals. Their lipophilic nature facilitates bioaccumulation in membranes leading to the generation of reactive oxygen species (SOR) that elicits oxidative damage in mammals. Rutin has shown pharmacological benefits including anti-tumor [6] and hepatic protective activities [7]. It is an antioxidant, comprised of the flavonol quercetin and the disaccharide rutinose. RT has inhibitory effects against membrane lipid peroxidation and generation of ROS [8]

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