Abstract
An isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method with ultra violet detection at 220 nm was used for a simple, selective and precise quantitation of a non-steroidal anti-inflammatory drug i.e. ibuprofen (IBF) from rat plasma. The procedure employed mefenamic acid as an internal standard (IS). A good chromatographic separation between IBF, the internal standard and interfering endogenous peaks was achieved using a Octadecyl Silane column and acetonitrile (ACN) - water - methanol - ortho-phosphoric acid (OPA) (in ratio of 58: 37: 5: 0.05 v/v/v/v) as the mobile phase at a flow rate of 1.0 ml/min. Two different extraction procedures for RP-HPLC quantitation of IBF were analysed. In the first method acetonitrile was used for extraction of the drug from plasma whereas the second method involved extraction of drug from plasma using diethyl ether. The second method proved to be more suitable for quantitation of the drug from plasma. The applicability of procedure includes the requirement of less sample volume, lesser organic solvent consumption and good plasma clean up for pharmacokinetic studies, in case of small animals, neonates and children as well.
Highlights
Ibuprofen (2-(4-isobutylphenyl) propionic acid) (IBF) (Fig. 1 a) is a widely prescribed non-steroidal antiinflammatory drug that shows antiinflammatory, analgesic and anti-pyretic properties1
The analgesic, anti-pyretic and antiinflammatory activity of non-steroidal anti-inflammatory drugs appears to be achieved mainly through inhibition of COX-2, whereas inhibition of COX-1 would be responsible for unwanted effects on platelet aggregation and the gastrointestinal tract2
HPLC grade Acetonitrile, water and methanol were purchased from Merck Chemicals (India), Ortho-phosphoric acid was obtained from Thermo-Fisher (India) and diethyl ether was obtained from Qualigen Ltd. (India)
Summary
Ibuprofen (2-(4-isobutylphenyl) propionic acid) (IBF) (Fig. 1 a) is a widely prescribed non-steroidal antiinflammatory drug that shows antiinflammatory, analgesic and anti-pyretic properties. Ibuprofen (2-(4-isobutylphenyl) propionic acid) (IBF) (Fig. 1 a) is a widely prescribed non-steroidal antiinflammatory drug that shows antiinflammatory, analgesic and anti-pyretic properties1 It is commonly used as an analgesic, especially in case of inflammation for relieving symptoms of arthritis, gout, fever and headache etc. Ibuprofen is a known non-selective cyclooxygenase (COX) inhibitor. This drug inhibits the COX enzyme and exerts its anti-inflammatory activity by inhibition of prostaglandin synthesis. The analgesic, anti-pyretic and antiinflammatory activity of non-steroidal anti-inflammatory drugs appears to be achieved mainly through inhibition of COX-2, whereas inhibition of COX-1 would be responsible for unwanted effects on platelet aggregation and the gastrointestinal tract.
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