A VALIDATED ANALYTICAL HPLC METHOD FOR THE QUANTIFICATION OF LINCOMYCIN HYDROCHLORIDE IN BULK AND SOLID DOSAGE FORM

P Rajeev Kumar,Rekha Rajeevkumar

doi:10.22159/ijap.2017v9i3.17327

Abstract

Objective: Develop a simple isocratic reverse phase high performance liquid chromatographic method (RP-HPLC) and validate for the determination of lincomycin hydrochloride (LMH) in bulk and pharmaceutical preparations.Methods: RP-HPLC quantification was carried out by using fine pack SIL RPC18 column. The mobile phase (methanol: water) was pumped at a flow rate of 1 ml/min in the ratio of 90:10 v/v and the eluents were monitored at 254 nm.Results: The retention time of the drug was 3.73 min and produced at a linear response in the concentration range of 5-25µg/ml. The percentage RSD was found to be below 2%. The LOD and LOQ were found to be 0.854µg/ml and 0.258µg/ml respectively.Conclusion: Validation of the method was performed for precision, accuracy, linearity, ruggedness, specificity and sensitivity to conform to ICH guidelines for valuation for analytical methods.

Highlights

Lincomycin hydrochloride (LMH) broadly used as a systemic antibiotic, belongs to the collection of lincosamide, which is effective against maximum common gram-positive bacteria
The linearity of lincomycin hydrochloride employing reverse phase high performance liquid chromatographic method (RP-HPLC) method was constructed by plotting area of lincomycin hydrochloride against concentration
Limit of detection (LOD) is calculated by using the formula 3.3 times σ/s where “σ” is the standard deviation of intercept and “s” is the slope obtained for calibration curve and limit of quantification was found to be 0.854μg/ml and 0.258μg/ml, respectively

Summary

Introduction

Lincomycin hydrochloride (LMH) broadly used as a systemic antibiotic, belongs to the collection of lincosamide, which is effective against maximum common gram-positive bacteria. Lincolnensis or by some other means[2,3]. It is approved in Indian pharmacopoeia [4, 5], British pharmacopoeia [6], the United States of pharmacopoeia and National formulary [7]. A few analytical method have been stated for its quantitative determination in pharmaceutical formulations like GC [4,5,6,7] liquid chromatography with pulsed electrochemical detection [8], UV spectrophotometry [9], colorimetry [10], and atomic absorption spectroscopy [11]. In view of the above fact, specific fast and sensitive analytical methods are in need for its quantitative determination

Methods

Results

Conclusion