Abstract
Objective: To develop a simple, sensitive, specific, accurate reverse phase high performance liquid chromatographic (RP-HPLC) method for the estimation of curcumin and piperine.Methods: The separation was done using a column Inertsil–ODS C18 (250 mm × 4.6 mm, 5µ particle size) and mobile phase composed of methanol: water (45:55 v/v), flow rate at 1 ml/min and detection was carried out at 282 nm with photodiode array (PDA) detector.Results: The separation of curcumin and piperine were found to be at the retention time of 2.433 min and 3.095 min, respectively. The method was found to be linear at a concentration range 20-80 µg/ml for curcumin and piperine. The limit of detection (LOD) and limit of quantification (LOQ) was found to be 0.05µg/ml and 0.17µg/ml for curcumin and 0.18µg/ml and 0.53µg/ml for piperine respectively. The average percentage recoveries of curcumin and piperine were in the range of 98-100.6%.Conclusion: A simple and sensitive reverse phase high performance liquid chromatographic method was developed for the estimation of curcumin and piperine.
Highlights
Curcumin is principal curcuminoid of indian spice turmeric of ginger Curcuma longa Linn
Based on literature it was confirmed that few HPLC methods were reported for simultaneous estimation of curcumin and piperine
The present method was simple, sensitive, and selective and developed with low-cost solvent in the mobile phase and simple standard preparation and separation were done at shorter run time and all parameters were validated according to ICH guidelines [25]
Summary
Curcumin is principal curcuminoid of indian spice turmeric of ginger Curcuma longa Linn. (family: Zingiberaceae). It has antioxidant, antiinflammatory, anti-fungal, anti-viral action [1] and anti-carcinogenic action [2]. Piperine was first pharmacologically active compound isolated from Piper longum and Piper nigrum (family: Piperaceae) [4]. Piperine(1-peperoyl piperidine) exhibits pharmacological activities like anti-hypertensive, anti-platelet [5], and anti-asthamatic, antitumor activities [6]. Literature survey revealed that few analytical methods were reported for the estimation of curcumin and piperine. Based on literature it was confirmed that few HPLC methods were reported for simultaneous estimation of curcumin and piperine. The present method was simple, sensitive, and selective and developed with low-cost solvent in the mobile phase and simple standard preparation and separation were done at shorter run time and all parameters were validated according to ICH guidelines [25]
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