Roles of notch signaling in rotenone-induced apoptosis of PC12 cells

Abstract

Objective To elucidate the roles of Notch Signaling in rotenone-induced apoptosis of PC12 cells.Methods PC12 ceils were treated with rotenone (5 μmol/L).Apoptosis of PC12 cells and activation of Notch signaling were observed.PC12 cells were pretreated with N-[N-(3,5-difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butvlester ( DAPT,10 μmol/L) before incubation with rotenone.The effect of Notch signaling on apoptosis of PC12 cells in response to rotenone was evaluated and the relationship between Notch signaling and rotenone-induced apoptosis of PC12 cells was analyzed.Results Rotenone significantly induced apoptosis of PC12 cells [( 35.6 ± 5.4 ) ％,P ＜ 0.05] and increased Caspase-3 activity (0.52 ±0.15,P ＜0.05 ).At the same time,rotenone activated Notch/Jagged1 signaling in PC12 cells.However,inhibition of Notch signaling by DAPT not only decreased apoptosis of PC12 cells [(9.8 ±3.1 ) ％,P ＜ 0.05],but also diminished Caspase-3 activity (0.16 ± 0.06,P ＜ 0.05 ).Conclusion Activation of Notch signaling could be one of the main mechanisms for rotenone-induced apoptosis of PC12 cells. Key words: Notch signaling; PC12 cells; Rotenone; Apoptosis