Role of MMP-2 (-1306 C/T) Polymorphism in Pituitary Adenoma.

Brigita Glebauskiene,Alvita Vilkeviciute,Giedrimantas Bernotas,Arimantas Tamasauskas,Rasa Liutkeviciene,Dalia Zaliuniene,Loresa Kriauciuniene

doi:10.1155/2016/2839697

Brigita Glebauskiene, Alvita Vilkeviciute + Show 5 more

Open Access

https://doi.org/10.1155/2016/2839697

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Journal: Scientifica	Publication Date: Jan 1, 2016
Citations: 47	License type: CC BY 4.0

Affiliation: Lithuanian University of Health Sciences

Abstract

Purpose. To determine if the frequency of the genotype of MMP-2 (-1306 C/T) Rs243865 has an influence on the development of pituitary adenoma (PA). Methods. The study enrolled n = 84 patients with PA and a random sample of the population n = 318 (reference group). The genotyping test of MMP-2 (-1306 C/T) was carried out using the real-time polymerase chain reaction method. Results. Analysis of MMP-2 (-1306 C/T) gene polymorphism has not revealed any differences in the genotype (C/C, C/T, and T/T) distribution between the PA patients and the reference group (as follows: 50%, 44%, and 6% versus 59.75%, 33.96%, and 6.29%). MMP-2 (-1306) C/C genotype was rarely observed in noninvasive PA compared to healthy controls: 35.1% versus 59.75%; p = 0.0049, as well C/C genotype being more frequently detected in nonrecurrence PA compared to healthy controls: 46.5% versus 59.75%; p = 0.0468. MMP-2 (-1306) C/T genotype was more frequently present in PA females compared to healthy controls females: 49.1% versus 33.66%; p = 0.041. Conclusion. Patients with noninvasive and nonrecurrence pituitary adenoma were the carriers of the C/C genotype significantly more frequently than their control counterparts and the C/T genotype in females was more frequent.

Highlights

Pituitary adenoma (PA) is a common benign monoclonal neoplasm accounting for approximately 15% to 20% of primary intracranial tumours [1]
Matrix metalloproteinases (MMPs)-2 (-1306) C/C genotype was rarely observed in noninvasive pituitary adenoma (PA) compared to healthy controls: 35.1% versus 59.75%; p = 0.0049, as well C/C genotype being more frequently detected in nonrecurrence PA compared to healthy controls: 46.5% versus 59.75%; p = 0.0468
MMP-2 (-1306) C/T genotype was more frequently present in PA females compared to healthy controls females: 49.1% versus 33.66%; p = 0.041

Summary

Introduction

Pituitary adenoma (PA) is a common benign monoclonal neoplasm accounting for approximately 15% to 20% of primary intracranial tumours [1]. The neoplastic process is associated with altered cell-stromal interactions that have a role in the morphogenesis of pituitary tumours [10]. Tumour cells must undergo several changes in molecular pathways in accordance with invasion-associated cellular activities, namely, cell-cell adhesion, cell-matrix adhesion and ectopic survival, migration, and proteolysis [11]. Matrix metalloproteinases (MMPs), a family of zinc-dependent endopeptidases, called matrixins, play an important role in the process of degradation of the extracellular matrix (ECM) and basement membrane (BM) in relation to tumour invasiveness, metastasis, and angiogenesis [12,13,14,15,16,17,18]. Many factors might induce MMPs production: cytokines, growth factors, physical stress, cell-extracellular matrix, and cell-cell interaction [19]

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