Abstract
Cultured Chinese hamster ovary cells were synchronized by mitotic selection. Relative synthesis rates for informosomal messenger-like RNA (mlRNA), polysomal messenger RNA (mRNA), and heterogeneous nuclear RNA (HnRNA) were estimated from the amount of labeled adenosine or uridine incorporated into these species in early and late interphase. The amounts of uridine incorporated into HnRNA, mRNA, and mlRNA during a pulse administered 9.75–10.75 h post-mitosis were 3.48, 4.64, and 2.82 times the amounts incorporated 1.5–2.5 h post-mitosis. Adenosine incorporation values 9.5–11.0 h post-mitosis were 1.64 (HnRNA), 2.49 (mRNA), and 1.18 (mlRNA) times the 1.5–3.0-h values. The relative incorporation into mRNA of large polysomes corresponded to incorporation into mRNA of small polysomes. Thus, the synthesis rates of mRNA, mlRNA, and HnRNA increase during interphase in a non-coordinate fashion.
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