Abstract
Simple SummaryThis is a novel study that explored the function of bta-miR-149-5p in the lipid metabolism of bovine adipocytes. Previously, we explored the role of bta-miR-149-5p gene in proliferation and differentiation of bovine adipocytes; however, the underlying mechanism of bta-miR-149-5p function in the regulation of lipid metabolism and adipogenesis in bovine adipocytes is unexplored. A transcriptomic study with integration of bioinformatics study was performed to fully explore the function of bta-miR-149-5p microRNA in bovine adipogenesis. This study will help the scientific community deal with lipogenesis for the breed improvement program of cattle for the provision of healthy meat to consumers. Intramuscular fat is a real challenge for the experts of animal science to improve meat quality traits. Research on the mechanism of adipogenesis provides invaluable information for the improvement of meat quality traits. This study investigated the effect of bta-miR-149-5p and its underlying mechanism on lipid metabolism in bovine adipocytes. Bovine adipocytes were differentiated and transfected with bta-miR-149-5p mimics or its negative control (NC). A total of 115 DEGs including 72 upregulated and 43 downregulated genes were identified in bovine adipocytes. The unigenes and GO term biological processes were the most annotated unigene contributor parts at 80.08%, followed by cellular component at 13.4% and molecular function at 6.7%. The KEGG pathways regulated by the DEGs were PI3K-Akt signaling pathway, calcium signaling pathway, pathways in cancer, MAPK signaling pathway, lipid metabolism/metabolic pathway, PPAR signaling pathway, AMPK signaling pathway, TGF-beta signaling pathway, cAMP signaling pathway, cholesterol metabolism, Wnt signaling pathway, and FoxO signaling pathway. In addition to this, the most important reactome enrichment pathways were R−BTA−373813 receptor CXCR2 binding ligands CXCL1 to 7, R−BTA−373791 receptor CXCR1 binding CXCL6 and CXCL8 ligands, R−BTA−210991 basigin interactions, R−BTA−380108 chemokine receptors binding chemokines, R−BTA−445704 calcium binding caldesmon, and R−BTA−5669034 TNFs binding their physiological receptors. Furthermore, the expression trend of the DEGs in these pathways were also exploited. Moreover, the bta-miR-149-5p significantly (p < 0.01) downregulated the mRNA levels of adipogenic marker genes such as CCND2, KLF6, ACSL1, Cdk2, SCD, SIK2, and ZEB1 in bovine adipocytes. In conclusion, our results suggest that bta-miR-149-5p regulates lipid metabolism in bovine adipocytes. The results of this study provide a basis for studying the function and molecular mechanism of the bta-miR-149-5p in regulating bovine adipogenesis.
Highlights
Adipose tissue stores energy in the form of neutral triglycerides and performs a vital function in maintaining energy homeostasis [1]
The transfected adipocytes were observed under fluorescence microscope, and the photographs were captured though Olympus IX71 microscope (OLYMPUS, Tokyo, Japan) (Figure 1B–D)
The expression trend of the Deferentially Expressed Genes (DEG) in these pathways were exploited. These data indicate the role of bta-miR-149-5p in adipogenesis through interactive regulation of DEGs and their respective KEGG pathways in bovine adipocytes
Summary
Adipose tissue stores energy in the form of neutral triglycerides and performs a vital function in maintaining energy homeostasis [1]. Adipocytes are the primary cell types of adipose tissue. There are two ways of adipose tissue development: one is hypertrophy (increase in adipocyte cell mass) and other is hyperplasia (increase in adipocyte numbers). In the case of surplus energy, the adipocytes deposits triglycerides (lipid droplets) through the lipogenic pathway, and in the case of energy deficiency, it releases lipids through the lipolysis pathway [2,3]. Selective improvement of IM fat without altering the other three adipose depots is very challenging in the meat industry [4]. The IM fat deposition contributes to the development of meat sensory qualities such as marbling, juiciness, flavor, and tenderness [5]. Approaches for the improvement of IM fat deposition are crucial for the development of meat quality. Research needs to be conducted to explore the underlying mechanisms of adipogenesis for the improvement of meat quality [6]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
