RmIL-33-stimulated nuocytes promote allergic inflammation in mouse model of allergic rhinitis

Abstract

Objective:The study aimed to investigate the role of nuocytes in allergic rhinitis （AR） murine models. Method:After intranasal administration of recombinant （rm） interleukin （IL）-33 in BALB/c mice, nuocytes were sorted and purified from the mouse nasal-associated lymphoid tissue （NALT）. Then, we examined the response of nuocytes to rmIL-33 in vitro. After a murine model of AR was established using ovalbumin, we adoptively transferred the cultured NALT-derived nuocytes to mice models, and determined allergic responses in them. Result:rmIL-33 expanded nuocytes in NALT of mice compared with AR mice （t=3.66, P<0.01）, and increased production of IL-13 from these cells in vitro in comparison with unstimulated nuocytes （t=19.90, P<0.000 1）. After adoptive transfer of nuocytes, sneezing （t=9.89, P<0.000 1） ,numbers of eosinophils（t=8.17, P<0.000 1）, concentrations of IL-13 （t=40.47, P<0.000 1） and IL-33 （t=19.89, P<0.000 1） in nasal lavage fluid were all enhanced when compared with AR mice. Conclusion:Nuocytes promote allergic inflammation in a murine model of AR.