Abstract

Objective:The study aimed to investigate the role of nuocytes in allergic rhinitis (AR) murine models. Method:After intranasal administration of recombinant (rm) interleukin (IL)-33 in BALB/c mice, nuocytes were sorted and purified from the mouse nasal-associated lymphoid tissue (NALT). Then, we examined the response of nuocytes to rmIL-33 in vitro. After a murine model of AR was established using ovalbumin, we adoptively transferred the cultured NALT-derived nuocytes to mice models, and determined allergic responses in them. Result:rmIL-33 expanded nuocytes in NALT of mice compared with AR mice (t=3.66, P<0.01), and increased production of IL-13 from these cells in vitro in comparison with unstimulated nuocytes (t=19.90, P<0.000 1). After adoptive transfer of nuocytes, sneezing (t=9.89, P<0.000 1) ,numbers of eosinophils(t=8.17, P<0.000 1), concentrations of IL-13 (t=40.47, P<0.000 1) and IL-33 (t=19.89, P<0.000 1) in nasal lavage fluid were all enhanced when compared with AR mice. Conclusion:Nuocytes promote allergic inflammation in a murine model of AR.

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