Abstract
BackgroundCD8+CD25+fork-head box transcription factor (Foxp3)+ regulatory T cells (CD8+ Tregs) play a role in immune tolerance. However, the role of these cells in allergic rhinitis (AR) has not been elucidated. The study aimed to evaluate influences of CD8+ Tregs on inflammatory conditions in a murine model of AR.MethodsA murine model of AR was established. CD8+ Tregs were isolated from mice nasal mucosa and cultured in vitro. We examined interleukin (IL)-10 and transforming growth factor (TGF)-β in cell cultures. Then, we administered CD8+ Tregs into mice nasal mucosal cultures, and examined eosinophil cation protein (ECP), IL-4, IL-5 and IL-13 in these cultures. Finally, we adoptively transferred CD8+ Tregs into mice models, and evaluated percentages of CD8+ Tregs, numbers of sneezing and nasal rubbing, and counts of eosinophils and contents of ECP, IL-4, IL-5, IL-13, IL-10 and TGF-β in nasal lavage fluid (NLF) in mice.ResultsThe percentage of CD8+ Tregs from AR mice was reduced. IL-10 and TGF-β were increased in cell cultures from AR mice. ECP, IL-4, IL-5 and IL-13 were decreased after the AR mice CD8+ Tregs administration in mucosal cultures. However, their contents were not changed after normal CD8+ Tregs treatment. Additionally, the adoptive transfer of AR CD8+ Tregs enhanced the percentage of CD8+ Tregs and levels of IL-10 and TGF-β in NLF, reduced numbers of sneezing and nasal rubbing, and counts of eosinophils and concentrations of ECP, IL-4, IL-5 and IL-13 in NLF. However, normal CD8+ Tregs could not change above parameters.ConclusionThese findings show that CD8+ Tregs may inhibit inflammatory responses in the AR condition.
Highlights
CD8+CD25+fork-head box transcription factor (Foxp3)+ regulatory T cells (CD8+ Tregs) play a role in immune tolerance
We used Tregs from nasal mucosa rather than peripheral blood mononuclear cells, just because these local cells were closer to their real allergic environment in allergic rhinitis (AR)
The results show that allergic C D8+ Tregs are likely to traffic into allergic nasal mucosa of mice from their tail veins through some unknown mechanisms
Summary
CD8+CD25+fork-head box transcription factor (Foxp3)+ regulatory T cells (CD8+ Tregs) play a role in immune tolerance. A similar survey over a 6-year period in the general Chinese adult population reports that the standardized prevalence of AR is characterized as specific IgE-mediated reactions against inhaled allergens driven by type 2 helper T (Th2) cells. This chronic condition results in nasal mucosal inflammation involving multiple cell types, such as dendritic cells, B cells, mast cells, eosinophils and basophils [5, 6], and various cytokines, such as interleukin (IL)-4, IL-5, IL-9 and IL-13 [7]. Immunoregulatory mechanisms, which may regulate the severity, persistence or alleviation of this chronic disease, are investigated further
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