Abstract
Vaccinia virus cores catalyze the incorporation of ATP into polyriboadenylic acid sequences of approximately 150 nucleotides in length. This incorporation of ATP occurs also in the presence of all four natural ribonucleotides in the reaction mixture. Poly A sequences are found in high molecular weight RNA synthesized by cores and evidence is presented for a covalent association between the poly A sequences and the regular viral RNA moiety.The transcription of poly dT sequences in vaccinia DNA appears to be the likely source of poly A, since about 1% of vaccinia DNA contains regions which hybridize with synthetic poly A and poly U. In addition, the synthesis of poly A is inhibited by proflavin sulfate and ethidium bromide, which complex with DNA. Poly A sequences are also synthesized in vivo during the growth cycle of vaccinia virus in HeLa cells.
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