Abstract
Summary The stage of arrest caused by 2-mercapto-l-(β-4-pyridethyl) benzimidazole (MPB) treatment was analyzed during replication of vaccinia virus in HeLa cells. 1) The reversal from MPB-block of viral RNA polymerase (a late enzyme) induction was prevented by the addition of hydroxyurea (HU). 2) MPB did not influence the induction of viral DNA polymerase (an early enzyme) and the switch-off control for it, but co-addition of HU with MPB permitted the super-induction of the enzyme synthesis. These results suggest that the process of vaccinia virus DNA synthesis for replication in HeLa cells may be divided into at least two stages, an MPB-sensitive and an MPB-resistant stage.
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