Rheumatoid Arthritis Patients With Circulating Extracellular Vesicles Positive for IgM Rheumatoid Factor Have Higher Disease Activity.

Onno J Arntz,Peter M Van Der Kraan,Peter L E M Van Lent,Marije I Koenders,Bartijn C H Pieters,Mark H Wenink,Fons A J Van De Loo,Frank H J Van Den Hoogen,Rogier M Thurlings

doi:10.3389/fimmu.2018.02388

Abstract

Rheumatoid arthritis (RA) is an autoimmune inflammatory disease that mainly affects synovial joints. Validated laboratory parameters for RA diagnosis are higher blood levels of rheumatoid factor IgM (IgM-RF), anti-citrullinated protein autoantibodies (ACPA), C-reactive protein (CRP) levels and erythrocyte sedimentation rate (ESR). Clinical parameters used are the number of tender (TJC) and swollen joints (SJC) and the global patient visual analog score (VAS). To determine disease remission in patients a disease activity score (DAS28) can be calculated based on SJC, TJC, VAS, and ESR (or alternatively CRP). However, subtle and better predictive changes to follow treatment responses in individual patients cannot be measured by the above mentioned parameters nor by measuring cytokine levels in blood. As extracellular vesicles (EVs) play a role in intercellular communication and carry a multitude of signals we set out to determine their value as a biomarker for disease activity. EVs were isolated from platelet-free plasma of 41 RA patients and 24 healthy controls (HC) by size exclusion chromatography (SEC). We quantified the particle and protein concentration, using NanoSight particle tracking analysis and micro-BCA, respectively, and observed no differences between RA patients and HC. In plasma of 28 out of 41 RA patients IgM-RF was detectable by ELISA, and in 13 out of these 28 seropositive RA patients (RF+RA) IgM-RF was also detected on their isolated pEVs (IgM-RF+). In seronegative RA patients (RF−RA) we did not find any RF present on pEVs. When comparing disease parameters we found no differences between RF+RA and RF−RA patients, except for increased ESR levels in RF+RA patients. However, RF+RA patients with IgM-RF+ pEVs showed significantly higher levels of CRP and ESR and also VAS and DAS28 were significantly increased compared to RA+ patients without IgM-RF+ pEVs. This study shows for the first time the presence of IgM-RF on pEVs in a proportion of RF+RA patients with a higher disease activity.

Highlights

Rheumatoid arthritis is an autoimmune disease in which the body’s immune system mistakenly attacks the synovial joints leading to joint inflammation as a hallmark feature of this disease [1]
In this study plasma extracellular vesicles (EVs) isolated from healthy controls an Rheumatoid arthritis (RA) patients did not show different characteristics in all parameters studied, in seropositive RA patients we have identified a subgroup expressing IgM-Rheumatoid factor (RF) on their pEVs and these patients showing significantly higher disease activity
Others found that the number of circulating EVs is enhanced in RA patients [24] but we could not confirm this result in our study

Summary

Introduction

Rheumatoid arthritis is an autoimmune disease in which the body’s immune system mistakenly attacks the synovial joints leading to joint inflammation as a hallmark feature of this disease [1]. Rheumatoid factor (RF) is found in 50% of early RA patients rising to 90% at advanced stages of disease [2]. Seropositive RA patients (RF+RA) have detectable RF in their circulation and it has been shown that high RF levels are predictors of more severe disease forms [3]. The pathogenesis of RA may be different between RF+RA and RF−RA patients with the later often reported as less severe studies are conflicting [4]. RF contribute to the disease process of RA by a mechanism where large immune complexes are formed and complement activation is induced [5]. Cardiovascular disease and organ involvement such as the lungs, heart, and eyes are wellrecognized complications occurring primarily in RF+RA patients [6]

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Conclusion