Abstract
An electrokinetic flow analysis system was introduced for reversed‐phase chromatography separation. It consisted of a C8‐modified silica monolithic column, a UV‐Vis detector, and a pressurizing electroosmosis pump (EOP) to deliver mobile phase steadily and continuously. Acetonitrile was used as the organic solvent component of the mobile phase, i.e., the pump carrier, to improve the separation resolution of three aromatic compounds. The effect of acetonitrile concentration on the separation resolution and flow rate of mobile phase was investigated. When 35% acetonitrile (v/v) solution, containing 0.5 mmol/L hexamethylene tetraamine, was used as the mobile phase, three aromatic components could be baseline separated by the electrokinetic flow analysis system.
Published Version
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