Abstract
The reversed-phase liquid chromatographic retention behavior of 33,500-Dalton and 75,000-Dalton poly(methyl methacrylate) (PMMA) and 26,000-Dalton to 913,000-Dalton poly(ethylene glycol) (PEG) samples are examined. It is found that isocratic retention can be obtained under carefully controlled experimental conditions, and the retention behavior of PMMAs and PEGs can be explained by conventional chromatographic models. Linear plots of the logarithm of the capacity factor (k') versus volume fraction (Φ) of the strong solvent are obtained for both PMMA and PEG samples. The slope (S) values in these plots are found to be in good agreement with S values calculated from two runs by gradient elution. S values from two gradient runs are used to successfully predict the retention time of the PMMA samples in a third gradient system.
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