RESVERATROL SIÇANLARDA OKSİDATİF STRESİ VE APOPTOZU BASKILAYARAK METOTREKSAT KAYNAKLI YUMURTALIK HASARINI HAFİFLETİR

Abstract

Objective
 The study aimed to evaluate the antioxidant and
 antiapoptotic effects of Resveratrol (RES) against
 Methotrexate (MTX)-induced ovarian damage in
 female rats and were divided into 7 groups:
 Material and Method
 Control; MTX (15mg/kg-1st day); MTX (1st and 3rd
 day); MTX (1st, 3rd and 5th day); MTX (1st day) +
 RES (20mg/kg); MTX (1st and 3rd day) + RES; MTX
 (1st, 3rd and 5th day) + RES. Since there was only
 one animal left in groups 4 and 6 and no animals
 in group 7, these groups were excluded from the
 evaluation. Ovarian damage was evaluated by
 performing hematoxylin-eosin (H-E) and TUNEL
 staining on ovarian tissues of rats. Total oxidant/
 antioxidant status in ovarian tissues was evaluated
 spectrophotometrically. In addition, DNA damage in
 blood was assessed by comet assay.
 Results
 As a result of biochemical analyzes, TOS levels
 increased in MTX groups, group 2 and 3, compared
 to the control group, while TOS level decreased
 significantly in group 5. On the other hand, TAS
 level increased significantly in group 5 compared
 to groups 2 and 3. H-E staining results showed that
 MTX-induced damage improved with RES (group 5)
 treatment. Maximum TUNEL positive staining image;
 it was observed in equal amounts in groups 2 and
 3, followed by group 5. In addition, the comet score,
 which indicates DNA damage, increased significantly
 in groups 2 and 3, while it decreased significantly in
 group 5.
 Conclusion
 In line with the results of this study, it was observed
 that Resveratrol may provide protective effects
 against Methotrexate-induced ovarian damage due to
 its antioxidant properties.