Response of light- and dark-grown callus of atrazine-resistant and susceptible rapeseed (Brassica napus) to varying concentrations of atrizine

Abstract

Callus cultures initiated with tissue of triazine-resistant and susceptible lines of Brassica napus L. were cultured in the presence of atrazine (10-5 to 10-4 M) under a 16-h photoperiod or in continuous darkness. On atrazine-free media callus growth was characteristically greater for the light-grown cultures. On media containing atrazine, growth of callus from triazine-resistant and susceptible rapeseed cultured in the dark did not differ significantly. Addition of atrazine to the culture medium resulted in a significant reduction in the growth of light-grown callus of the susceptible genotype to a level equivalent to that of the dark-grown cultures. Atrazine had no effect on the growth of light-grown callus initiated with triazine-resistant rapeseed.