Abstract
The genetics/inheritance of callus induction and growth in terms of gene action, genetic variance components, and allied genetic parameters was determined in five pure lines of rapeseed (Brassica napus L.), Orakel, ACSN1, P504588, P704591 and Boanty by diallel analysis of F1 progenies from five parents. The parents and their F1 hybrids were grown on MS medium in Petri dishes. The data were collected after five weeks on percent of callus induction, callus diameter and callus fresh weight. The additive (and dominance (Ĥ) components were significant for all traits. H1 and H2 were higher than D, indicating that, the dominant component was larger than additive component. Epistasis was almost absent. Dominant alleles caused high and recessive alleles caused low expression of callus induction and growth. Because of better breeding value, high per-se performance, and desirable position in the Wr –Vr graph, three parents Orakel, ACSN1 and Boanty were identified as potential genotypes to be exploited for callus growth improvement by hybridization followed by selection. Key words: Callus growth, diallel analysis, inheritance, rapeseed, dominant alleles.
Highlights
Brassica napus is one of the world's most important sources of vegetable oil and protein, B. napus has become an object of extensive tissue culture (Turgut et al, 1998) and suspension culture (Biesaga-Koscielniak et al, 2008; Kharenko et al, 2011) studies and breeding
The absence of epistasis primarily confirmed the adequacy of the Hayman's additive model for the analysis of genetic variance and a-allied parameters the two tests of additively, namely F(wr-vr) which was significant for fresh weight, diameter of callus and percentage of callus induction and bWr/Vr being > 0.5 and insignificant for all traits revealed absence of non-allelic interactions in controlling gene expression (Table 2)
One of the assumptions underlying diallel analysis is the absence of epistatic gene action which has been satisfied for percent of callus induction, diameter of callus and fresh weight of callus in this study
Summary
Brassica napus is one of the world's most important sources of vegetable oil and protein, B. napus has become an object of extensive tissue culture (Turgut et al, 1998) and suspension culture (Biesaga-Koscielniak et al, 2008; Kharenko et al, 2011) studies and breeding. In vitro characters can be used in combination with other agronomically important traits for crop improvement programs. This approach requires knowledge of the genetic basis for ‘in vitro aptitudeand will help to predict the response of these characters to selection (Calligari et al, 1985; Powell et al, 1985). The genetic basis of in vitro response has been analyzed in several plant systems including alfalfa (Wan et al, 1988; Kielly and Bowley, 1992; Crea et al, 1995), barley (Foroughi-Wehr et al, 1982; Komatsuda et al, 1989; Özgen et al, 2005), sunflower (Encheva et al, 2004), cotton (Gawel and Robacker, 1990), maize
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