Abstract
AbstractCallus induction and subculture was successful with mature embryos and stem sections of seedlings of Zea mays L. on Linsmaier and Skoog's medium modified to contain 4 mg/I of 2,4‐D and 1 g/I of casamino acids. — 2,4‐D was superior to NAA and IAA for both callus induction and growth. Callus subcultured on NAA formed abundant roots on agar‐solidified media and numerous root‐like primordia in liquid cultures. — Kinetin had no effect on callus induction in the presence of 2,4‐D and neither kinetin nor gibberellic acid stimulated callus growth during subculture. — Callus grew equally well on the medium of Linsmaier and Skoog, that of Schenk and Hildebrandt, and the B‐5 medium of Gamborg and Eveleigh containing 2% sucrose, 4 mg/I of 2,4‐D and 1 g/I of casamino acids. — The callus grew more rapidly at 25°C than at 30°C or 35°C. Little difference was noted at any temperature in callus growth in alternating light (16 h) and dark (8 h) or continuous dark. — Sucrose was superior to glucose and maltose in both liquid and agar‐solidified cultures. Lactose and galactose failed to support callus growth.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
