Response of dominant plant species to periodic flooding in the riparian zone of the Three Gorges Reservoir (TGR), China

Abstract

Human-induced disturbances such as dam construction and regulation often change the duration and frequency of flooding and thus notably influence plant dominance in riparian zones. Even though numerous studies have indicated that the oxidative stress and antioxidative stress systems are essential for plant defenses against adverse flooding stress, the mechanism of vegetation distribution due to hydrological regimes is still unclear. In the current study, the riparian zone of the Three Gorges Reservoir (TGR), which experiences seasonal and anti-seasonal water-level fluctuations, was used to investigate the dominant species. To our knowledge, this is the first study that links molecular-physiological-morphological mechanisms to explore the development of flooding tolerance of dominant riparian species. Physiological traits (e.g., chlorophyll and protein contents), morphological traits (e.g., leaf length), and molecular traits (e.g., enzymatic antioxidant activity and the malondialdehyde content) were analyzed at different water-level gradient zones of the dominant species to evaluate the influence of flooding. To explore the regulation mechanisms of submergence for the vegetation distribution, correlation analysis, PCA (principal component analysis) and laboratory flooding experiments were conducted. The results showed that Cynodon dactylon, which has a rapid antioxidative system, was the dominant species in the riparian zone of the TGR. The leaf length varied significantly along with water level gradients (p < 0.05) with the minimum values appearing in the lowest part of the riparian zone and the maximum values observed in the highest areas. The chlorophyll and protein contents fluctuated in different water level gradient zones, but significant differences were not observed. Within the antioxidative system, catalase was found to be essential for riparian plants in their response to flooding. The current study could provide insight to explore the specific mechanism of resistance for dominant plants to periodic flooding, and the reason why dominant species can survive adverse stress.