Abstract
PurposeTo investigate the influence of implant surface decontaminated and uncontaminated on osteoblast-like cell adhesion and proliferationMaterials and methodsCommercially available implants of different brands and surface characteristics were selected: Biomet 3i® Nanotite (NT) and Osseotite (OT), Straumann® SLActive (SLA), and Neodent® Acqua Drive (ACQ) and Neoporos Drive CM (CM). Physical and chemical properties of the implants were investigated by scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDS), and wettability analysis (WETT). Implants were previously contaminated with Aggregatibacter actinomycetemcomitans strains; after that, samples were decontaminated by different chemical methods. Decontaminated (test group; n = 15/type of implant) and uncontaminated (control group; n = 5/type of implant) samples were analyzed according to the number of human osteoblastic osteosarcoma cells (Saos-2) adhered on the implant surface after 24 h and 72 h in SEM images.ResultsACQ was found to be highly hydrophilic, and NT was the most hydrophobic implant. Increased variation of Saos-2 cell adhesion and proliferation were observed on all test and control groups. Controversially, at the proliferation analysis in 72 h, CM implant was the only implant that showed no significant difference between test and group (p = 0.2833; Tukey’s multiple comparisons test). NT implants showed the greater value of cell proliferation when compared with all types of implant surface (p = 0.0002; Tukey’s multiple comparisons test).ConclusionsThese findings suggest that decontaminated surfaces were able to impair the counting of osteoblast-like cell adhesion and proliferation.
Highlights
Osseointegration is defined as a direct contact between vital bone and implant surface at optical microscopy level [1] and describes a rigid fixation of an alloplastic material in the bone, in an asymptomatic clinical situation and functional load [2]
These findings suggest that decontaminated surfaces were able to impair the counting of osteoblastlike cell adhesion and proliferation
NT and Neoporos drive Cone Morse (CM) implants were composed of titanium only (100%); OT implant showed the presence of titanium
Summary
Osseointegration is defined as a direct contact between vital bone and implant surface at optical microscopy level [1] and describes a rigid fixation of an alloplastic material in the bone, in an asymptomatic clinical situation and functional load [2]. Just like in natural teeth, the development of biofilm induces biological responses of hard and soft tissue. Balderrama et al International Journal of Implant Dentistry (2020) 6:84 around implants, resulting in pocket deepening, bleeding on probing, exudation, bone loss, and clinical mobility, impairing the success of implant therapy [8, 11]. The colonization, structure, and composition of the biofilm on implant surfaces is influenced by its surface roughness, chemical composition, hydrophobic properties, surface electrical charge, and energy [7, 12]. Modifications in micro- and nano-topography of dental implants were proposed to increase bone-to-implant contact, but biofilm accumulation in rougher surfaces is accelerated when implant threads are exposed to the oral cavity [12, 13], which make these areas more difficult to decontaminate [13]. Moderate rough implants show higher rates of reosseointegration when compared to machined ones [14]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
