Research on the Functional Microbe Activation System in a Post-Polymer Flooded Reservoir

Abstract

Further exploitation of the residual oil underground in post-polymer flooded reservoirs is attractive and challenging. Microbial-enhanced oil recovery (MEOR) is a promising strategy to enhance the recovery of residual oil in post-polymer flooded reservoirs. Identifying and selectively activating indigenous microorganisms with oil displacement capabilities is an urgent requirement in the current design of efficient microbial-enhanced oil recovery technologies. This study combines high-throughput sequencing with functional network analysis to identify the core functional microbes within the reservoirs. Concurrently, it devises targeted activation strategies tailored to oligotrophic conditions through an analysis of environmental factor influences. The feasibility of these strategies is then validated through physical simulation experiments. With nutrient stimulation, the overall diversity of microorganisms decreases while the abundance of functional microorganisms increases. The core displacement results showed that the oil recovery factor increased by 3.82% on the basis of polymer flooding. In summary, this research has established a system for the efficient activation of functional microorganisms under oligotrophic conditions by utilizing bioinformatics, network analysis, and indoor simulation systems. This achievement will undoubtedly lay a solid foundation for the practical implementation of microbial enhancement techniques in the field.