Research on Mechanism of FGFR1 Inhibitor BAY1163877 against Proliferation of Breast Cancer Cells

Abstract

To investigate the effect and mechanism of fibroblast growth factor receptor 1 (FGFR1) inhibitor BAY1163877 on proliferation and apoptosis of breast cancer cells. The expression of FGFR1 in human breast cell lines was detected by qRT-PCR and western blot. IC50 of BAY1163877 and cell viability were measured by CCK-8 method. Cell proliferation was observed by colony assay. Cell apoptosis after treatment of BAY1163877 was tested by flow cytometry. The expressions of p-FGFR1/FGFR1 and p-STAT3/STAT3 protein were detected by Western blot. According to the results of qRT-PCR and Western blot, FGFR1 high expression of breast cancer cell line MDA-MB-231 and FGFR1 low expression of breast cancer cell line MCF-7 were selected. BAY1163877 inhibits proliferation of MDA-MB-231 and MCF-7 cells and induces apoptosis of MDA-MB-231 and MCF-7 cells. The results of Western blot showed that the expression of p-FGFR1 and p-STAT3 protein in MDA-MB-231 was reduced after BAY1163877 treatment and the expression in MCF-7 was not significantly changed. BAY1163877 inhibits the proliferation and induces the apoptosis of high FGFR1 expression breast cancer cell line MDA-MB-231, and its mechanism may be related to the decrease of p-FGFR1 and p-STAT3 protein expression. BAY1163877 inhibits the proliferation and induces the apoptosis of MCF-7, but its mechanism still needs the further study.