The expression of thyroid cancer-1 in breast cancer and its effect on proliferation and apoptosis of breast cancer cells

Abstract

Objective To investigate the expression of thyroid cancer-1 (TC-1) in breast cancer and its influence on the proliferation and apoptosis of breast cancer cells. Methods Reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting were used to detect the expression of TC-1 in breast cancer tissues and corresponding paracancerous tissues. The breast cancer cell MCF-7 were transfected with TC-1 siRNA and siRNA control, it was named as interference group and negative group, the cells without treatment were used as control group. Cell proliferation was detected by methyl thiazol tetrazolium (MTT), apoptosis was detected by flow cytometry, the expressions of β-catenin, c-Myc, Cleaved Caspase-3 were detected by Western blotting. Results The level of TC-1 in breast cancer tissues was higher than that in paracancerous tissues (t=17.746, P=0.000; t=12.705, P=0.000). The apoptosis rate, survival rate, TC-1, β-catenin, c-Myc, Cleaved Caspase-3 in the control group and the negative group did not change significantly. The rate of apoptosis in the interference group was higher than that in the control group, the cell survival rate was lower than that of the control group, and the levels of β-catenin and c-Myc were lower than those of the control group, the levels of Cleaved Caspase-3 was higher than those of the control group (t=13.986, P=0.000; t=7.015, P=0.002; t=10.457, P=0.001; t=27.886, P=0.000; t=7.426, P=0.002). Conclusion The expression of TC-1 was up-regulated in breast cancer tissues, interference with TC-1 can inhibit proliferation of breast cancer cells, promoting apoptosis of breast cancer cells, the Wnt signaling pathway may be one of its mechanisms. Key words: Breast cancer; Proliferation; Apoptosis; Thyroid cancer-1