Abstract
In the study, Lactobacillus plantarum 18-2, Leuconostoc citreum 37 and Leuconostoc citreum 39, which were isolated from Inner Mongolia traditional dairy products, were used to detect their biofilm formation ability by the micro-titer plate biofilm assays. The results showed that the biofilm-forming ability of the three strains differed along with the alteration of their nutritional and environmental conditions. The optimal biofilm formation conditions of L. plantarum 18-2 were in pH5.6 MRS broth without any additives for 12 h at the temperature of 30°C Otherwise, L. citreum 37 was reported the best biofilm formation ability, when it’s cultivated in pH 5.4 for 48 h at 30°C, while the culture in pH5.5 for 48 h at 30°C is most conducive to the biofilm forming of L. citreum 39. In addition, when sodium chloride or sucrose was added to MRS broth, the biofilm forming was inhibited as the concentration increased. Thus it can be seen that, L. plantarum and L. citreum 37 and 39 were capable of forming biofilm and the biofilm formation was strongly modulated by culture conditions and medium, including low pH; low temperature and high osmolality.
Highlights
Attaching to the biological or non-biological surface, biofilm consists of bacterial colonies and coated bacteria (Hall-Stoodley et al, 2004) and is widely applied in nature and industrial production, such as fermentation industry and sewage treatment system (Van Schaik and Abee, 2005)
Biofilms of Pseudomonas aeruginosa were more resistant to heavy metals than planktonic bacteria (Teitzel and Parsek, 2003); Biofilms of Burkholderia cepacia and of P. aeruginosa were more resistant to antibiotics (Kubota et al, 2008)
It’s reported that the BF formation process can be divided into 3 phases: 0-6 h adhesive period, 6-24 h for agglomeration period and 24-72 h for mature period. In accordance to such principles, the study set up a timeline for BF formation to inspect BF forming conditions
Summary
Attaching to the biological or non-biological surface, biofilm (hereinafter referred to as BF) consists of bacterial colonies and coated bacteria (Hall-Stoodley et al, 2004) and is widely applied in nature and industrial production, such as fermentation industry and sewage treatment system (Van Schaik and Abee, 2005). There were few reports have demonstrated the formation of lactic acid bacteria biofilms. Lactic Acid Bacteria (LAB) are widely recognized to be the safest one applied to the food industry. Lactic acid is one of major materials for the food, pharmaceuticals and daily chemical industries; lactic acid bacteria bacteriocins can be used as the natural protective agent for foods, which do no harm to body health, and have great nutritive value. Some lactic acid bacteria are the key risk factors for food deterioration. The study on BF formation plays a significant role in improving LAB suitability in fermentation agent production and preventing food materials, semi-finished and finished products from decaying
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