Research of antimicrobial efficacy of a composition with prolonged antiseptic effect against planktonic and film forms of clinical strains of non-fermentative Gram-negative bacteria

Abstract

Annotation. The problem of treatment of burn wounds is closely related to the specificity of the spectrum of microorganisms that impair wound healing in this category of patients. A generally alarming trend in recent years is the significant increase in the etiological structure of wound infection, both in frequency and in severity and in the difficult choice of treatment tactics in the isolation of poly antimicrobial resistant strains of Pseudomonas aeruginosa, Acinetobacter baumannii. The aim of our study was to create a model of biofilm formed by bacteria on the wound surface and to investigate, in a comparative study, the sensitivity of planktonic and film forms of non-enzymatic gram-negative bacteria to a composition based on decamethoxin with prolonged antiseptic action. To determine the effect of the antiseptic composition on planktonic and film forms of bacteria there was used the method of two consecutive dilutions. The results of a comparative study of the activity of the antiseptic composition with decamethoxin planktonic and biofilm forms of strains of NFGB indicated that in the bacterial film bacteria were more resistant to drugs than in planktonic form. Film forms of A. baumannii and B. cepacia have a sensitivity to decamethoxin at a concentration of 13.6±1.95 µg/ml and 15.6±0 µg/ml, respectively. P. aeruginosa film forms have a higher degree of resistance to the test composition with decamethoxin (MBc 137.5±30.6 μg/ml). Disinfection of the planktonic form of NFGB is 10 times faster than the film form, the destruction of which is achieved with prolonged exposure of 120–150 minutes.