RESEARCH OF ANTIMICROBIAL ACTIVITY OF ANTHELMINTHIC MIXTURE FOR NEMATODOSIS TREATMENT

Kateryna Semchenko,Kateryna Romas

doi:10.21303/2585-663.2017.00456

Kateryna Semchenko, Kateryna Romas

Open Access

https://doi.org/10.21303/2585-663.2017.00456

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Abstract

Parasitic infections are one of the most acute problems of the modern society as this group of diseases is met in all countries of the world. According to the World Health Organization around 1.2 billion people are infected with diverse parasitic infections each year. Helminthiases of digestive system, primarily nematodosis, constitute a group of special risk, since a particularly sensitive audience for them are children of younger and middle age. Phytotherapy, which is based on the centuries-old experience of folk medicine, promises to be a solution to this problem, enabling to create effective complex medicines for nematodosis prevention and treatment. Thus, the herbal mixture was prepared on the basis of the offered composition of medicinal plant raw material. The research of antimicrobial activity of the studied herbal mixture was conducted in vitro by the method of diffusion into agar at the Biotechnology Department of the National Pharmaceutical University under the direction of Doctor of Pharmaceutical Sciences, prof. Strelets O.P. The obtained results of the carried out research of the antimicrobial properties of the herbal mixture samples allowed examining the level of antimicrobial activity. The further research of the investigated herbal mixture and the offered phytocomposition is promising for creation of new effective medicines with the complex action including anthelminthic (specific to nematodes), antiparasitic, anti-inflammatory, antibacterial, antiseptic, hepatoprotective, nephroprotective, laxative, antiallergic, antispasmodic, analgesic, sedative activities

Highlights

Helminths in humans are classified into round, tape and flukes
The following pure cultures were used as the test cultures: gram-positive microorganisms Staphylococcus aureus ATCC 25293, spore culture Bacillus subtilis ATCC 6633, gram-negative cultures Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853
It should be noted that the samples revealed weak antimicrobial activity in relation to the test culture of Staphylococcus aureus: the diameters of growth inhibition are less than 15 mm, namely 13.6±0.5 mm

Summary

Introduction

Helminths in humans are classified into round (nematodes), tape (cestodes) and flukes (trematodes). The toxic effect of the products of vital activity and disintegration of helminths parasitizing in the human body causes damage to the nervous system, dyspeptic disorders, suppression of the secretory and motor functions of the digestive system. Nematodes (causative agents of ascariasis; trichocephalosis; enterobiosis; ankylostomidosis; strongyloidiasis; trichinosis) are one of the most common groups among helminths of the digestive system (Table 1) [3]. The centuries-old heritage of folk medicine in the treatment of digestive system helminthiases contains many recipes for monoand polycomponent remedies. The analysis of this experience, as well as drugs offered by phytomedicine, made it possible to identify a number of medicinal plant raw material, which is promising for the creation of complex herbal medicines with polyvalent pharmacological activity [6, 7]

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