Abstract
Velocity sedimentation at high ionic strength, of DNA released from M13-infected bacteria lysed under gentle conditions, provides a simple method for separating phage-specific DNA components from bacterial DNA. 1. 1. Components sedimenting at rates of 0.71 and 0.57 times that of viral single strands are identified as component I replicative form (RFI-DNA) and component II replicative form (RFII-DNA), respectively. 2. 2. The major phage DNA component early ( <30 min ) in the infectious process is RFI-DNA; at later times ( > 45 min ) the major phage DNA component is intracellular single strands. 3. 3. The source of intracellular single strands is a pool of free DNA molecules rather than progeny phage particles.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
