Abstract
DNA of the domestic chicken, Gallus domesticus, was fractionated by sonication, denaturation and reassociation on hydroxyapatite column. The reassociated repetitious DNA fraction comprises 17% of the genome and showed 2 distinct peaks in neutral CsCl density gradients. In situ hybridization using 3 H-RNA complementary to the various repetitive DNA fractions showed that the microchromosomes and the W chromosome had a disproportionately high content of repetitive DNA and that the two types of chromosomes contained different fractions of repetitive DNA.
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