Abstract
Nuclear DNA from wheat, rye, and Triticale plantlets was isolated and purified. Purity of the samples was tested spectrophotometrically. Tm values and hyperchromicities were determined for the three DNA samples. The DNA(s) were denaturated and annealed under controlled conditions. Cot curves were carried out for total DNA on the three samples and for the repetitive DNA fractions.The analysis of those curves indicated that the genomes of wheat and rye are made of highly repetitive, intermediate, and unique DNA fractions. The highly repetitive fraction (107) is suggested as corresponding to palindrome areas in the genome.The Triticale genome showed a similar pattern to the parents, except for the highly repetitive DNA fraction which showed different orders of repetitiveness.
Published Version
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