Release of Ca2+ by noradrenaline and ATP from the same Ca2+ store sensitive to both InsP3 and Ca2+ in rat portal vein myocytes.

Abstract

1. Changes in cytosolic free Ca2+ concentration ([Ca2+]i) induced by noradrenaline (NA) and ATP were investigated using indo-1 microspectrofluorimetry in single smooth muscle cells of rat portal vein. 2. Activation of P2x-purinoceptors by ATP (10 microM) increased [Ca2+]i from 92 +/- 7 nM (n = 18) to 557 +/- 30 nM (n = 11). In the presence of NA (10 microM), the ATP-induced rise in [Ca2+]i was reduced to 23.6 +/- 1.5% (n = 7) of the control response (in the absence of NA). 3. Tetracaine (10 microM to 2 mM) inhibited in a concentration-dependent manner the Ca(2+)-induced Ca2+ release (CICR) evoked by 5 mM caffeine. In the presence of 1 mM tetracaine, the rise in [Ca2+]i induced by ATP (10(-8)-10(-4) M) was strongly inhibited. A tetracaine-resistant rise in [Ca2+]i, corresponding to 26.4 +/- 2.3% (n = 14) of control values, was recorded in response to 10 microM ATP. 4. The amplitude of the NA-induced [Ca2+]i rise depended on NA concentrations (10(-8)-10(-5) M) and was not modified by tetracaine (1 mM). 5. This study suggests that Ca2+ ions released through the InsP3 receptor-channel upon NA application do not activate CICR and the InsP3- and Ca(2+)-sensitive Ca2+ store appears to represent, at least functionally, a single releasable Ca2+ pool.