Abstract
Relative amounts of free and bound forms of several gibberellins (GA3 GA4, GA7, GA9) were determined in dormant and in after-ripened embryos of Pyrus malus L. cv. Golden delicious. We used a modified lettuce hypocotyl test applied after two thin-layer chromatographies. The latter allow a good separation of the different gibberellins studied from indol-acetic acid and abscisic acid. In order to avoid imbibition of the embryos before extraction, we submitted them to an after-ripening treatment inside the fruits. We showed that small quantities of free and bound gibberellins are present in the dormant embryos, mainly located in the cotyledons. In the embryonic axes of the after-ripened embryos, we found significant quantities of free GA9, and a very high level of bound GA4, whereas cotyledons contained small quantities only of the free and bound gibberellins tested. The significance of the large quantity of bound GA4, its possible origin and mobilization are discussed.
Published Version
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