Relationship between HBV genotypes/sub-genotypes of hepatitis B virus and antiviral therapy

Abstract

Objective To investigate the relationship between the genotype/sub-genotype and antiviral treatment. Methods The clinical data of 159 patients with chronic HBV infections were collected, who were treated by adefovir dipivoxil(10mg/d)or entecavir(0.5mg/d)according to the disease's conditions. The genotypes and subtypes of hepatitis B virus were determined by Nested polymerase chain reaction(nt PCR). The levels of serum HBVDNA replication, ALT levels and HBV serologic markers were detected pre or post-treatment 24 weeks,48 weeks. Observed the relationship between the HBV genotypes/sub-genotypes and the antiviral efficacy of adefovir dipivoxil or entecavir treatment. Results After 24 weeks of adefovir dipivoxil therapy,ALT normalization rate of subgenotype Ba and subgenotype C2 was 66.7%vs 66.2%(χ2=0.74,P>0.05),HBeAg negative conversion rate of two subgenotypes was 27.3%vs 23.1%(χ2=0.10,P>0.05),HBVDNA negative conversion rate of two sub genotype Ba and subgenotype C2 was 33.3%vs 30.9%(χ2=0.03,P>0.05),respectively.After treatment for 48 weeks,ALT normalization rates of subgenotype Ba and subgenotype C2 were 83.3%vs 82.4%(χ2=0.01,P>0.05),HBeAg negative conversion rates of two subgenotypes were 45.5%vs 34.4%(χ2=0.49,P>0.05),HBVDNA negative conversion rates of two subgenotypes were 58.3%vs 48.5%(χ2=0.39,P>0.05).After 24 weeks of entecavir therapy,ALT normalization rates of subgenotype Ba and subgenotype C2 were 71.4%vs 69.6%(χ2=0.02,P>0.05),HBeAg negative conversion rates of two subgenotypes were 33.3%vs 30.8%(χ2=0.03,P>0.05),HBVDNA negative conversion rates of two subgenotypes were 42.9%vs 39.3%(χ2=0.06,P>0.05),respectively. After treatment for 48 weeks,ALT normali zation rates of subgenotype Ba and subgenotype C2 were 85.7%vs 83.9% (χ2=0.03,P>0.05),HBeAg negative conversion rates of two subgenotypes were 50.0%vs 44.9%(χ2=0.10, P>0.05),HBVDNA negative conversion rates of two subgenotypes were 71.4%vs 67.8%(χ2=0.07,P>0.05). Conclusion The study showed that genotype C(C2)is a predominant HBV genotype among people with chronic HBV infections in Shanxi province.HBV subgenotypes Ba and C2 have no significant difference in virologic, biochemical, and immunologic response to ADV or ETV. Key words: Chronic Hepatitis B; Genotype; Adefovir Dipivoxil; Entecavir