Abstract
A new organic Se compound, 2‐hydroxy‐4‐methylselenobutanoic acid (SeO), was shown to be more bioavailable than Na2SeO3 or seleno‐yeast (SeY) to chicks, hens, and pigs. This study was to determine regulations of 26 selenoprotein genes, 5 selenoproteins (GPX1, GPX4, SEPP1, SEPW1, and SELU), and 5 selenocystenine biosynthesis and degradation‐related genes (Pstk, SeciSbp2, Sephs1, SepSecS, and Scly) by the three sources of Se in liver, breast, thigh, and/or plasma of chicks. Day‐old male broilers (n = 6 cages/diet, 6 chicks/cage) were fed a Se deficient, corn/soy‐based diet (BD, 0.05 mg Se/kg) or the BD supplemented with Na2SeO3, SeY, or SeO at 0.2 mg Se/kg for 6 weeks. While the three sources of Se enhanced (P < 0.05) mRNA levels of Gpx1, Gpx3, Sepp1, Pstk, and SepSecS in all three tissues over the BD group, only SeO upregulated (P < 0.05) the expression of Sels and Sepx1 in the liver and thigh. Compared with Na2SeO3, SeO caused greater increases (P < 0.05) in expression of Gpx3 mRNA and GPX4, SELU, and SEPP1 proteins in the three tissues and/or plasma. A greater (P < 0.05) expression of GPX4 and SEPP1 protein in the three tissues and/or plasma and Sepp1 and SepSecS mRNA in the two muscles was produced by SeO than SeY. In conclusion, SeO exhibited a unique ability to induce the expression of Sels and Sepx1 mRNA and to enhance the production of GPX4 and SEPP1 proteins in the chick tissues.Support or Funding InformationSupported in part by NSFC 31501987 and 31320103920, NSTSPC‐2013BAD20B04, and Adisseo.
Published Version
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