Vitamin D3 inhibits invasion and migration of lung cancer A549 cells via nuclear factor-κB/GPX1 pathway

Abstract

Objective To observe the effect of Vitamin D3 on the invasion and migration of lung cancer A549 cells and to explore its mechanism. Methods A549 cells were treated with Vitamin D3 at a concentration of 60 nmol/L for 5 d, and 10 μmol/L BAY11-7082 for Ah cells for 1h. A549 cells were transfected with small interfering RNA (siRNA) targeting GPX1, and A549 was detected by Transwell method. The ability of cells to invade and migrate was detected by Western blotting to detect the expression levels of nuclear factor-κB (NF-κB) and GPX1 proteins. Results After treatment with Vitamin D3, the migration and invasion ability of A549 cells decreased significantly [P(Blank)<0.01; p(AA)<0.05], and the expression of NF-κB and GPX1 decreased. After treatment with NF-κB inhibitor BAY11-7082, the migration and invasion ability of A549 cells decreased significantly (P<0.01), and the expression of NF-κB and GPX1 was observed to decrease. After the expression of GPX1 was decreased by RNA interference, the migration and invasion ability of A549 cells decreased significantly [P(Blannk)<0.01, P(Scramble)<0.05], but there was no significant change in NF-κB. Conclusion Vitamin D3 can inhibit the invasion and migration of lung cancer A549 cells through NF-κB/GPX1 pathway. Key words: RNA interference; Glutathione peroxidase-1; Vitamin D3; Non-small cell lung cancer