Abstract
Abstract A simple staining procedure was developed for differentiating plant virus inclusions in epidermal strips. A solvent utilizing methyl Cellosolve as a major constituent was used as a vehicle for three staining combinations: calcomine orange-“luxol” brilliant green, congo rubin-methyl green, and phloxine-methylene blue. The strips were studied by mounting either in dilute stain or in Euparal. The procedure was used to stain inclusions formed by the following viruses: tobacco mosaic, tobacco etch, clover yellow mosaic, potato Y, tomato aucuba mosaic, potato X, bean yellow mosaic, bean common mosaic, watermelon mosaic, and sugar cane mosaic. These procedures were found valuable in selecting material for study with the electron microscope and in diagnosing plant virus infections.
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