Abstract
Partial purification of some elongated plant viruses was facilitated by the use of ether and carbon tetrachloride for preliminary clarification of infectious plant juice. When equal parts of juice and peroxide-free ether were emulsified by shaking, noninfectious colloidal components of the juice, such as chloroplasts, were coagulated. After the emulsion was broken by centrifugation, the clarified aqueous phase containing the virus was removed and freed from ether by shaking with carbon tetrachloride. Further purification steps included differential and rate-zonal densitygradient centrifugations. Partially purified preparations were serologically active and as far as tested infectious. They were used as antigens in immunological studies with the viruses of: bean common mosaic, bean yellow mosaic, pea streak, white clover mosaic, carnation latent virus, potato viruses M, S, X, and Y. Rabbits were injected intravenously with saline suspensions of antigen, and intramuscularly with an emulsion of antigen in Freund's adjuvant. The antibody titre of each animal's serum was then determined periodically. When Freund's adjuvant was used antibody titres were higher, and periods of high antibody response lasted longer than when adjuvant was not used. During these periods it was not possible to increase the antibody production appreciably by booster injections even though the type of injection was different from that used for primary immunisation. In some cases, antiserum titres over 1:106 were obtained.
Published Version
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