Abstract
A rapid, inexpensive method has been derived that allows the simultaneous determination of Protoporphyrin and Mg-Porphyrins in the same extract.The procedure allows for accurate (> 90%), repeatable estimates in the nanomolar range.Analyses of experimental conditions known to alter porphyrin metabolism, such as illumination of etiolated material or incubation in 5-aminolevulinic acid, give results that are consistent with those reported by others. Using this procedure, it is shown that one characteristic of chilling induced chlorosis involves a non-lethal alteration in the ability of etiolated tissue to synthesize chlorophylls.
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